IN FOCUS: DIFFERENTIATION OF EMBRYONIC NEURONAL PROGENITORS AND GLIA CO-CULTURES
3DProSeed ® is the ideal synthetic hydrogel platform to study differentiation and maturation of human neuronal progenitor cells (NPCs) and induced pluripotent stem cells (iPSCs).
We offer protocols of neuronal differentiation of human embryonic neuronal progenitors and development of co-cultures with human primary astrocytes.
Our focus on neuronal differentiation and glia co-cultures
Establishment of human astrocytic cultures
Astrocytes are glial cells in the brain and spinal cord involved in the maintenance, support and repair of the nervous system. Numerous researchers suggested that culturing primary astrocytes in a 3D hydrogel environment helps to better mimic the glial status in the undamaged nervous system. The reason is that the hydrogel culture maintains the astrocytes in a less reactive state compared to when cultured as monolayers on standard TCPs.
We offer to our clients simple protocols to culture human primary astrocytes in our synthetic 3DProSeed hydrogel plate and to study their extra-cellular matrix protein deposition.
Culture and differentiation of human neuronal progenitors
We offer protocols to expand and differentiate human neural progenitor cells derived from NIH-approved H9 human embryonic stem cells (hESCs) in the 3DProSeed hydrogel plate. We also propose protocols to expand and differentiate neuronal stem cell lines derived from developing human brain transduced with the myc transcription factors, displaying high phenotype and genotype stability as well as multipotential neuronal differentiation capacity over long-term culture.
These cultures can be developed in our fully synthetic hydrogels under serum-free conditions, and can be co-cultured with pre-seeded mesenchymal supporting cells and astrocytes.
These cultures can be used for studies and high-content screenings in neurotoxicity, neurogenesis, electrophysiology, neurotransmitter and receptor functions.