3DProSeed ® Hydrogel Well - Plate
Pre-assembled synthetic hydrogels in a microtiter plate for ready-to-use and automation-compatible cell-based assays in 3D. The patented 3DProSeed® hydrogel surface enhances the penetration of cells.
Simply seed cells on the hydrogel to establish a 3D culture.
No hydrogel assembly step
The hydrogels in the plate are hydrated
ready for cell seeding
Fully synthetic
poly(ethlyene glycol)-based
For the highest control over the culture conditions
Sequential Seeding
of various cell populations at different time points
of your assay for complex co-cultures
KEY FEATURES
3DProSeed® Hydrogel Plate
Our 3DProSeed® microtiter plate combines the advantages of hydrogel-based cultures with the simplicity and workflow integration of standard polystyrene cultureware.
Our plate contains pre-assembled synthetic hydrogels, delivered to you hydrated, ready for cell seeding.
The hydrogels are fully synthetic made of state-of-art poly(ethlyene glycol)-based bioconjugates. This hydrogel material offers the highest level of control over the culture conditions and optical transparency for high quality microscopy.
The key innovation is the 3DProSeed hydrogel surface featuring an in-depth density gradient which enables penetration of seeded cells into the bulk.
Our special surface makes it possible to sequentially seed various cell populations in the same gel to establish complex co-cultures.
SPECIFICATIONS
1. The plate
Standard ANSI footprint 96-well black imaging plate with 180 microns glass bottom.
Plate sealed with a polyproylene adhesive foil and covered with its standard polystyrene lid.
2. The Hydrogel
Pre-assembled hydrogels at the bottom of each well. Hydrogels delivered hydrated in a storage buffer ready for cell seeding.The special inner-well geometry for facilitated manual pipetting.
Optically clear synthetic hydrogels made of poly(ethylene glycol) bio-conjugates.
Basic formulation contains cell adhesion sites and protease-sensitive degradation sites. Advanced formulations are available depending on the application of interest.
3. Patented hydrogel in-depth density gradient
The hydrogel gradually disappears in the proximity of the upper surface becoming water.
This special surface enhances penetration of cells seeded and enables repeated seeding of various cell populations for the development of advanced co-culture models in the gel.
RESEARCH APPLICATIONS
Oncology and Immuno-oncology
Highlights
Tumor cells cultured in a pre-established stromal microenvironment made of fibroblasts, mesenchymal stromal cells, adipocytes or endothelial cells.
Sequential addition of immune cells to complex tumor cultures for killing assays and IO studies.
Neurosciences
Highlights
Culture of human astrocytes, human progenitors and iPSCs-derived neurons and neuronal differentiation in the gel.
Co-cultures of iPSC-neurons with astrocytes and microglia via sequential seeding.
Phenotypic assays on microglia monocultures.
Stem Cells Culture and Differentiation
Highlights
Culture of human primary mesenchymal stem cells.
Adipogenic and osteogenic differentiation.
Angiogenesis
Highlights
Culture of human primary mesenchymal stem cells with endothelial cells.
Interaction of cancer cells with the vascular niche.